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Procédé d'extrusion réactive et/ou enzymatique pour l'extraction de phycocolloïdes : application aux produits de la mer ArchiMer
Makoure, Dounia; Arhaliass, Abdellah; Echchelh, Adil; Baron, Regis; Legrand, Jack.
In this paper we present the reactive extrusion process to extract the phycocolloids from the sea products. By comparing with other techniques, this process has a several advantages like the economic return, the flexibility, the production of various products with different characteristics and time gains. There are two types of extruder: single-screw and double-screw, each one has its specific properties. The solid products are introduced by means of the hopper that is equipped with a dosage pump for the regulation of the flow. Liquid products are, generally, injected in the not filled zones, through an external pump. The products undergo thermo mechanical modifications and then the shaping of the final products was done in the die. The reactive extrusion...
Tipo: Text Palavras-chave: Extrusion réactive; Valorisation; Coproduits; Produits de la mer; Phycocolloïdes alginate; Reactive extrusion; Valorization; By-products; Sea-products; Phycocolloids; Alginate.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00500/61171/64645.pdf
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Lay and scientific categorizations of new breeding techniques: Implications for food policy and genetically modified organism legislation ArchiMer
Debucquet, Gervaise; Baron, Regis; Cardinal, Mireille.
The rapid development of new genetic breeding techniques is accompanied by a polarized debate around their risks. Research on the public perception of these techniques lags behind scientific developments. This study tests a method for revealing laypeople’s perceptions and attitudes about different genetic techniques. The objectives are to enable laypeople to understand the key principles of new genetic breeding techniques and to permit a comparison of their modes of classification with those of scientific experts. The combined method of a free sorting task and focus groups showed that the participants distinguished the techniques that did not induce any change in DNA sequence, and applied two different logics to classify the other breeding techniques: a...
Tipo: Text Palavras-chave: Food policy; Genetically modified organism regulation; Genetically modified organisms; Lay categorization; New breeding techniques; And public understanding.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00634/74604/74979.pdf
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Numerical and experimental study of a continuous electrostatic smoking process ArchiMer
Baron, Regis; Havet, Michel; Solliec, Camille; Pierrat, Daniel; Touchard, Gerard.
The smoking process is a batch one with long duration processing (some hours). Deposition of smoke particles can be enhanced by electrostatic precipitation. However, a better knowledge of the fundamental physics is needed to optimize the process. We carried out experimental and numerical studies to obtain essential information on the smoke flow. First, experiments physically characterized the wood smoke. These measures lead us to study the turbulent two-phase flow using an algebraic slip model. The main coupling with electrical forces is taken into account through the slip velocity. Various assumptions were tested concerning the coupled phenomena in order to build an algorithm that is able to simulate these phenomena. Numerical resolutions are compared...
Tipo: Text Palavras-chave: Smoked salmon; Smoke particles; Electrostatic precipitators.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4695.pdf
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Modélisation de la contamination des bivalves par les toxines d'Alexandrium spp. Rapport final ArchiMer
Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie.
L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs.
Tipo: Text
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf
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Optimisation d'un nouveau procédé de fumage du poisson par précipitation électrostatique des particules de fumée ArchiMer
Baron, Regis; Sanquer, Odile; Département Sciences et Techniques Alimentaires Marines de l'IFREMER de Nantes; UMR CNRS GEPEA; CETIM; UMR LEA Univ. Poitiers.
Cette étude porte sur l'optimisation d'un nouveau procédé de fumage en continu par précipitation électrostatique des particules de fumée. Le principe est de faire circuler Ia fumée, produite par pyrolyse de bois, entre deux électrodes. Sous des conditions électriques spécifiques, un flux ionique entre les électrodes est généré.
Tipo: Text
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00065/17626/15155.pdf
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Etude de l'extrusion ArchiMer
Baron, Regis.
Ce rapport fait suite au stage effectué au sein du laboratoire «Génie Alimentaire», de l'Institut Français de Recherche pour l'Exploitation de la MER. Ce stage fait partie du cycle de préparation à la Maîtrise de Sciences et Techniques en Systèmes Automatiques Industriels. Il s'est déroulé du 25 Février au 17 Mai 1991.Ce rapport porte sur l'étude du procédé d'extrusion.
Tipo: Text
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00137/24870/22958.pdf
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Improving detoxification Efficiency of PSP-contaminated oysters (Crassostrea gigas Thunberg) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp.
Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large...
Tipo: Text Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf
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Use of random forest methodology to link aroma profiles to volatile compounds: application to enzymatic hydrolysis of Atlantic salmon (Salmo salar) by-products combined with Maillard reactions ArchiMer
Cardinal, Mireille; Chaussy, Marianne; Donnay-moreno, Claire; Cornet, Josiane; Rannou, Cecile; Fillonneau, Catherine; Prost, Carole; Baron, Regis; Courcoux, Philippe.
To use salmon protein hydrolysates as food ingredients and to mask the fish odor, Maillard reactions were associated with enzymatic production of hydrolysates. The study explored an original approach based on regression trees (RT) and random forest (RF) methodologies to predict hydrolysate odor profiles from volatile compounds. An experimental design with four factors: enzyme/substrate ratio, quantity of xylose, hydrolysis and cooking times was used to create a range of enzymatic hydrolysates. Twenty samples were submitted to a trained panel for sensory descriptions of odor. Hydrolysate volatile compounds were extracted by means of Headspace Solid Phase MicroExtraction (HS-SPME) and analyzed using gas chromatography/mass spectrometry (GC-MS). The results...
Tipo: Text Palavras-chave: Sensory characteristics; Volatile compounds; HS-SPME/GC-MS; Regression tree; Random forest; Hydrolysate; Maillard reactions.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00624/73590/73024.pdf
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Elements for optimizing a one-step enzymatic bio-refinery process of shrimp cuticles: Focus on enzymatic proteolysis screening ArchiMer
Baron, Regis; Socol, Marius; Kaas, Raymond; Arhaliass, A.; Rodriguez Del Pino, J.; Le Roux, Karine; Donnay-moreno, Claire; Berge, Jean-pascal.
This article complements an earlier work published in 2015 Baron et al. (2015) that showed the interest of a shrimp shells bio-refining process. We compare here the effect of eleven commercial proteases at pH 3.5 or 4.0 on a residual amount of shrimp shells proteins after 6 hours at 50 °C. The two pH are obtained when respectively 40 and 25 mmol of formic acid are added to 5 g of mild dried shell. Deproteinisation yield above 95% are obtained. Residual amino acids profile in the solid phase was identical for the eleven proteases except for pepsin which was similar to the raw material profile. A significant relative increase in the proportion of Glycine is observed for the ten other cases. Likewise, shapes of size exclusion chromatograms of the dissolved...
Tipo: Text Palavras-chave: Bio-refinery; Shrimp cuticles; Acidic enzymatic proteolysis.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49957/50530.pdf
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Fabrication d'un aliment piscicole frais et humide à base de captures accessoires et de coproduits de poissons ArchiMer
Doudard, Karine; Chim, Liet; Arhaliass, Abdellah; Trintignac, Pascal; Garsi, Pierre; Legrand, Jack; Baron, Regis.
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00605/71719/70182.pdf
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Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides ArchiMer
Vandanjon, Laurent; Vallet, Laurent; Le Glatin, Titouan; Deleris, Paul; Baron, Regis; Bourseau, Patrick; Dumay, Justine.
The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.   After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial...
Tipo: Text Palavras-chave: Brown macroalgae; Sargassum muticum; Enzymatic hydrolysis; Surfactants; Phlorotannins.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00350/46105/46073.pdf
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A Physiologically Structured Equation to Consider Quota Heterogeneity in the Droop Model ArchiMer
Mairet, Francis; Baron, Regis.
The Droop model allows to represent microalgae growth limited by a nutrient, using a cell quota (also referred to as variable-yield) approach. Single-cell measurements have revealed quota heterogeneity in phytoplankton collected from field studies. Such heterogeneity can be due, among other factors, to spatial structure (e.g. in biogeochemical cycles in the ocean, or for photobioreactors connected in series). Nonetheless, quota heterogeneity is generally omitted in modelling studies, using an average quota approach, or included in size-structured or individual-based models. Here, we propose a distributed Droop equation to tackle this problem, considering subpopulation growth -in line with Droop macroscopic view- rather than cell division dynamics. We...
Tipo: Text Palavras-chave: Population Balance Model; Variable-yield model; Cell Quota; Phytoplankton; Microalgae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00600/71187/69542.pdf
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Gut passage times in two bivalve molluscs fed toxic microalgae: Alexandrium minutum, A-catenella and Pseudo-nitzschia calliantha ArchiMer
Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent.
The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (...
Tipo: Text Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf
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Paralytic shellfish poison outbreaks in the Penze estuary: Environmental factors affecting toxin uptake in the oyster, Crassostrea gigas ArchiMer
Lassus, Patrick; Baron, Regis; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Leguay, Didier; Amzil, Zouher.
Several experiments using a self-regulated system were conducted to define the factors likely to influence the uptake of paralytic shellfish poison (PSP) by oysters in the Penze estuary (France, Brittany). Each 4-day experiment was carried out in a recirculated sea water system using 15 Pacific oysters (Crassostrea gigas) separated from each other and supplied with unfiltered natural seawater containing alternatively toxic (Alexandrium minutum) or non-toxic (Skeletonema costatum) algal diets. The food supply and exposure times to toxic diets were determined according to field studies of the Upstream and downstream movement of patches containing A. minutum. The experimental parameters corresponded roughly to the hydrological conditions generally observed in...
Tipo: Text Palavras-chave: Experimental contamination; Oyster toxicity; Dinophyceae; Toxin; PSP.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-400.pdf
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Odor Modification in Salmon Hydrolysates Using the Maillard Reaction ArchiMer
Kouakou, Christelle; Berge, Jean-pascal; Baron, Regis; Lethuaut, Laurent; Prost, Carole; Cardinal, Mireille.
The aim of this work was to study the effect of adding sugar during proteolysis to promote the Maillard reaction and mask the initial fish odor and off-flavors generated. An experimental design, based on the Doehlert plan, was used to study the influence of hydrolysis conditions (time, temperature, sugar, and antioxidant addition) on the odor characteristics of hydrolysates, soluble protein levels, and amino acid content. Results showed that the lowest level of sugar (10g of D-xylose added to 1kg of by-products) was enough to develop a grilled odor in hydrolysates. In the hydrolysis conditions used, i.e. enzyme inactivation at 95°C for 30min, hydrolysis temperature had no effect on grilled odor production but significantly affected the soluble protein...
Tipo: Text Palavras-chave: Salmon by-product; Hydrolysate; Maillard reaction; Sensory characteristics; Off-flavor; Soluble protein.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00156/26702/24778.pdf
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Modélisation de la contamination des huîtres par les toxines d'Alexandrium minutum - Rapport final (2ème année) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille.
L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer).
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf
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Utilisation des sous-produits de la pêche et de l'aquaculture pour l'alimentation en aquaculture ArchiMer
Penven, Anais; Baron, Regis; Etienne, Monique; Delannoy, Charles; Berge, Jean-pascal.
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00607/71938/70635.pdf
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Dynamical modelling of a reactive extrusion process: Focus on residence time distribution in a fully intermeshing co-rotating twin-screw extruder and application to an alginate extraction process ArchiMer
Baron, Regis; Vauchel, Peggy; Kaas, Raymond; Arhaliass, Abdellah; Legrand, Jack.
The context of this study is the modelling of reactive extrusion process based on an alginate extraction protocol. Residence Time Distribution (RTD) is one important part to predict the kinetics of reactive compounds. A simple model is proposed to predict RTD in fully intermeshing co-rotating twin-screw extruders without reaction. This model, which can be easily extended to reactive case in a future work, is based on the extension of an axial dispersion model, including control parameters (screw speed and flow rate) and geometrical parameters (screw profile and die design). Simulations were performed for various operating and geometrical conditions so as to illustrate possibilities offered by the proposed model. Validation was conducted for two different...
Tipo: Text Palavras-chave: Alginate; Extraction; Extrusion; Mathematical modelling; Residence time distribution; Simulation; Laminaria digitata.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11592/8985.pdf
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Modelling of paralytic shellfish toxin biotransformations in the course of Crassostrea gigas detoxification kinetics ArchiMer
Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Truquet, Philippe; Haberkorn, Hansy; Lassus, Patrick; Barille, Laurent; Amzil, Zouher.
The aim of this study was to evaluate the importance of biotransformation of paralytic shellfish toxins during the detoxification process in contaminated oysters. Mathematical models based upon the detoxification patterns of digestive gland and other tissues were developed. It was demonstrated that biotransformations do not seem to play an important role in digestive gland or other tissue detoxification kinetics with our data set. Moreover, different toxin transfers from digestive gland toward other tissues were investigated. No significant transfer was highlighted in our data set. These first conclusions were drawn after comparing the results obtained from 13 biotransformations and identifiable transfer scenarios. Finally, to determine a more robust...
Tipo: Text Palavras-chave: Detoxification kinetics modelling; Biotransformation of saxitoxin analogues; Oyster.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00049/16047/14309.pdf
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Continuous fluorescence recording as a way to improve Pacific oyster (Crassostrea gigas) models of paralytic shellfish toxin accumulation ArchiMer
Baron, Regis; Couedel, Marielle; Joret, Camille; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Lassus, Patrick.
A simple system was used to simulate the effect of alternating toxic (paralytic shellfish poisoning toxins) and non-toxic microalgal diets on oyster feeding behaviors and rates of toxin accumulation. These experimental conditions were meant to reflect, to some extent, the incoming and outgoing fluxes of toxic algae observed at the mouth of the Penze estuary (Northern Brittany, France). Physiological and toxicological parameters were estimated based oil fluorescence measurements recorded continuously at the outlet of each experimental tank, which contained a single oyster. Q(tox), this variable describes toxin uptake in oysters, it was used (instead of the toxin ingestion rate): i) in simple graphical analyses, ii) as well as in one- and two-compartment...
Tipo: Text Palavras-chave: Skeletonema costatum; Alexandrium minutum; Fluorescence; Modeling; Kinetics; Toxin uptake; Shellfish toxin; PSP.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1400.pdf
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